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To enhance the thermal stability and affinity of the developed RFX001.D , the same group later engineered variant RFX037.D by the strategic incorporation of additional amino acids at their N- and C-termini, guided by the higher affinity observed in a bacterially expressed variant of RFX001 containing an N-terminal His10 tag 77

The abundances of UCG-010 and UCG-005 significantly decreased, while Selenomonas abundance significantly increased

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