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Two possibilities are that DHA concentrations are extremely low in compartments such as the chloroplast and cytosol or that rate constants for the chemical reaction are much slower in the crowded cellular environment than those measured in dilute solutions in vitro, making enzymes necessary to favour the reaction between DHA and GSH

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Such co-expression to produce separate proteins can be achieved, for example, using an internal ribosome entry site (IRES) or a self-cleaving peptide to link the genes [47]