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The supernatant was collected using a micropipette and stored in Eppendorf tubes at 80 C in the refrigerator for various biochemical tests

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PMID: 99 IraniMMerhiZ

The cells were washed with serum-free medium three times to remove excess DCFH-DA and immediately analysed using the FACSCanto II flow cytometer

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Dimerization and antidepressant recognition at noradrenaline transporter