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We advise using the area around the belly button if it has some fat

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Mitochondria-targeted therapies, including antioxidants [such as, coenzyme Q10, N-acetylcysteine (NAC) and -lipoic acid] and mitochondrial nutritional supplements, have emerged as promising alternatives aimed at mitigating oxidative stress and restoring mitochondrial function

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Mass Spectrometry: Data-dependent MS/MS analysis was performed using a Q Exactive Orbitrap mass spectrometer (Thermo Scientific)

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After incubation, the batches were centrifuged at 4000 g for 25 minutes and the pellets were re-suspended in lysis buffer (200 mM NaCl, 50 mM Tris-HCl, 5 mM EDTA, 2 mM benzamidine, 1 mM phenylmethylsulfonylfluoride and 0.5 g/l lysozyme, pH 7.8)

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