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RNA extraction and RT-qPCR Total RNA was extracted from frozen specimens and kidney cancer cell lines using TRIzol reagent (9108, Takara, Japan) and cDNA was generated using Evo M-MLV Reverse Transcription Premix Kit (AG11728, Accurate Biology, China)

intro Tirzepatide is a dual GIP and GLP-1 receptor agonist manufactured by Eli Lilly, available under two brand names: Zepbound (FDA-approved for chronic weight management and obstructive sleep apnea) and Mounjaro (FDA-approved for type 2 diabetes)

However, challenges like low tumor uptake, off-target effects, and potential resistance remain, underlining the need for further refinement in nanoparticle design and delivery systems

Copper plays a role in collagen-related processes, and GHK-Cu has been researched in laboratory settings for its interaction with skin and follicle-related pathways

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The characterization of feces and urine: a review of the literature to inform advanced treatment technology