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These splenorenal APSC are thought to prevent the spleen from undergoing congestion, 17 which is why splenomegaly is not a feature of canine pre- and intrahepatic portal hypertensive disorders

Oxidative stress can lead to oxidative damage of proteins, lipids, and nucleic acids in cells and then death of DA neurons

The scrambled-shRNA sequence (gttcttctcggtagatgtaataattattacatctaccgagaagaacc) was taken from Surgical procedures For the treatment with Gsto2- shRNA or scrambled shRNA, mutants were randomly selected from ear-notch littermates and assigned to the two experimental groups

Doering, Faculty of Health Sciences, Department of Pathology and Molecular Medicine, McMaster University, HSC 1R1, 1280 Main Street West, Hamilton, ON L8S 4K1, Canada e-mail: [email protected] This article was submitted to the journal Frontiers in Cellular Neuroscience

we observed a strong growth defect and massive cell death in the LHON-edited cells, but not in the SILENT-edited cells (population doublings per day: LHON 0.64 0.11, SILENT 0.52 0.07