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As limited in current reference reports, more human data and toxicological investigation is needed to further elucidate the adverse actions of prenatal APAP exposure to offspring

For spleen-derived suspensions, red blood cells were additionally removed by incubation with ACK lysis buffer (R1010, Solarbio) according to the manufacturers protocol before washing

After addition of loading buffer, lysates were vortexed, and supernatants were heated to 95 C for 5 min

10.1016/j.lfs.2019.117066 146 JangJ.JeongH.JangE.KimE.YoonY.JangS.et al (2023)

Using expired reconstituted solution means injecting a product of unknown potency and questionable sterility

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