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Bioreactor temperature during infection was investigated to maximize titer, and a decrease in temperature from 37C to 34C yielded a twothree-fold increase in infectivity

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MM/PBSA (Molecular Mechanics/Poisson-Boltzmann Surface Area) calculates the free energy difference between the ligand and the protein-ligand complex in various states, which helps evaluate the binding affinity of a ligand to a protein

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Finally the levels of protein carbonyls, as an oxidative stress marker, were detected

CuNPs group: Mice were injected intraperitoneally with 0.2 ml (0.667 mg/kg b.w.) of CuNPs started from the seventh day of the experiment daily for 2 weeks (5 times each week)