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The membranes were washed 3 times for 5 min each with TBST and then incubated with Peroxidase AffiniPure Goat Anti-Mouse IgG (H + L) secondary antibody (1:10000, Jackson ImmunoResearch) in 5% BSA in TBST for 1 h at room temperature

3 Vitamin B12 deficiency The haematological effects of B12 deficiency are the same as for folate deficiency, and are characterised by macrocytic anaemia (with abnormally large red blood cells)

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While some users may not report any noticeable changes immediately, some have noticed increased energy, and signs of detoxification

Tian, C