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Mice were euthanized and blood was collected by cardiac puncture and processed immediately for simultaneous determination of EtOH and acetaldehyde by headspace gas chromatography/mass spectrometry (HS-GC/MS) analysis

Antibody information Primary antibodies used were RB (Cell Signaling Technology, # 9309, 1:1000), RB (Cell Signaling Technology, # 9313, 1:1000), E2F1 (Proteintech, #66515-1-Ig, 1:1000), Phospho-Rb (Ser795) (Cell Signaling Technology, # 9301S, 1:1000), RBL1 (Proteintech, #13354-1-AP, 1:1000), RBL2 (Proteintech, #27251-1-AP, 1:1000), Cyclin B1 (ABclonal, # A19037, 1: 1000), RNF40 (ABclonal, # A6443, 1: 1000), LRPPRC (ABclonal, # A3365, 1: 1000), CDK4 (ABclonal, # A11136, 1: 1000), CDK6 (ABclonal, # A0106, 1: 1000), KATNA1 (ABclonal, # A16491, 1: 1000), c-MYC (ABclonal, # A1309, 1: 1000), KLHL42 (Invitrogen, # PAS-54292, 1: 500), Vinculin (Santa Cruz, # sc-73614, 1:1000), -Actin (Cell Signaling Technology, # 4970, 1:1000), Myc (Santa Cruz, # sc-40, 1:1000), HA (Cell Signaling Technology, # 3724, 1:1000), Flag (MBL, #M185-7, 1:1000), Rabbit IgG (ABclonal, # AS014, 1:3000), Mouse IgG (ABclonal, # AS003, 1:3000)

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