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10.1016/s0306-4522(00)00526-1 363 VaradeJ.ComabellaM.OrtizM

The Material: One 10mg vial of lyophilized AHK-CU

Petkov et al., JCI 2003

Animal tissues were lysed with RIPA lysis buffer (50 mM Tris, 150 mM NaCl, 1% Triton X-100,1% sodium deoxycholate, 0.1% SDS, 5 mM sodium orthovanadate, 5 mM sodium fluoride, 1 mM and EDTA, pH 7.4) supplemented with 10 l/ml protease inhibitor cocktail (GE Healthcare Biosciences, Pittsburgh, PA, United States)

The mean absolute bioavailability of tirzepatide following subcutaneous administration is 80%

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