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Injection Process

After in vitro stimulation with CpG, B cells from older individuals produce significantly lower levels of IgM and IgA antibodies compared to younger individuals, highlighting a diminished capacity to respond to novel pathogens while maintaining responsiveness to frequently encountered antigens [357]

Bacterial cells exposed to 30 ppm of AgNPs for 2 h were pelleted at around 3,700 rpm for 12 min ( n = 3), washed with phosphate-buffered saline (PBS, 1, pH 7.4), and imaged

Together, these components constitute the extracellular matrix (ECM) that determines skin's mechanical properties, hydration, and appearance

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Cryo-EM data were acquired on a 300 kV Titan Krios electron microscope (Thermo Fisher Scientific) equipped with a K3 Summit direct electron detector (Gatan) mounted after a quantum energy filter set to a slit width of 20 eV