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Samples were fixed in 10% buffered formalin overnight at 4C, dehydrated with increasing concentrations of ethanol, embedded in paraffin, cut into 5 m sections, and stained with hematoxylin and eosin (HE) or Massons Trichrome Stain Kit (Sigma-Aldrich)

5.4 Clinical evidence: melatonin in ART cycles and PCOS-related infertility Clinical data on melatonin as an adjunct in assisted reproduction (and specifically in PCOS) converge on a plausible benefit for intermediate outcomes, with more cautious signals for hard endpoints