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Tissue was then cryosectioned coronally at 20 C into 24 well plates (ThermoFisher Scientific) containing PBS 0.1% sodium azide at 25 m thickness and stored at 4 C until use

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Indeed, lithium therapy of cultured cerebellar granule cells increased mRNA and protein levels of Bcl-2, and the Bcl-2/Bax protein level ratio increased by 5-fold after treatment duration for 57 days 124

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