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Cellular and organelle membranes are enriched with polyunsaturated fatty acids (PUFAs), which act as primary substrates for lipid peroxidation ( During lipid remodeling, two core enzymesacyl-CoA synthetase long-chain family member 4 (ACSL4) and lysophosphatidylcholine acyltransferase 3 (LPCAT3)drive PUFA-dependent ferroptosis ( As essential components of cell membranes, PUFAs further play a pivotal role in regulating retinal lipid metabolism and exerting antioxidant effects

In terms of the immune response, our secretome analysis revealed enrichment of immune-related pathways under oxidative stress, with qPCR confirming increased expression of inflammatory mediators IL-6, IL-11, and IL-18 shortly after H 2 O 2 treatment

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Clinics that use numbing creams may ask the patient to apply it at home before attending the appointment