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Role of nitric oxide and superoxide in allergen-induced airway hyperreactivity after the late asthmatic reaction in guinea-pigs

Briefly, coverslips were washed with washing buffer A, probed with anti-mouse PLUS and anti-rabbit MINUS 1:500, where PLUS and MINUS stands for 5 to 3 reverse and forward oligonucleotides, respectively, and incubated for one hour at 37C