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Notably, Hcy, choline, cystathionine, methionine, and taurine played a significant role in the probabilistic principal component analysis model

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For example, the multienzyme complex pyruvate dehydrogenase [7] at the junction of glycolysis and the citric acid cycle requires five organic cofactors and one metal ion: loosely bound thiamine pyrophosphate (TPP), covalently bound lipoamide and flavin adenine dinucleotide (FAD), cosubstrates nicotinamide adenine dinucleotide (NAD + ) and coenzyme A (CoA), and a metal ion (Mg 2+ )

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Moreover, it acts as a lubricant, aids air humidification, and provides a selectively permeable barrier for gas exchange and nutritional absorption [5, 6]