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Treatment with Centella asiatica extract inhibits the expression of interleukin-6 (IL-6) and tumor necrosis factor- (TNF-) in a dose-dependent manner in inflammatory HaCaT cells, stimulated by interferon- (IFN-) and TNF--triggered inflammation

TLR4, Toll-Like Receptor 4

& Kondratov, R

For use in vivo studies, the liver tissues were homogenized in a buffer at 4 C using a homogenizer (#Polytron CH-6010, Kinematica GmbH, Luzern, Switzerland)

The molecular docking and dynamics studies revealed that DSF formed a stable complex with PIK3 via interactions with residues Ile932, Ile800, Ile848, Met772, and Trp780, which contributed significantly to stability

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