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Extrusion bioprinting A technique used to create organized tissue constructs by continuously dispensing or extruding a bioink through a nozzle, resulting in 3D structures

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Equal amounts of proteins underwent 10% sodium dodecyl sulfatepolyacrylamide gel electrophoresis (Solarbio) for separation, and were transferred to polyvinylidene difluoride membranes (0.45 m, Millipore, Billerica, MA, USA)