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The disulfide in the active site of Trxs can subsequently be reduced by a dedicated reductase, i.e., Trx reductase (TrxR), at the expense of reduced nicotinamide adenin dinucleotide phosphate (NADPH)

The ex vivo fluorescence images further confirmed that DiR@DssD-Hb group had much higher Cy5-miRNA accumulation in tumor site compared with free Cy5-miRNA group

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Gene expression levels were obtained by RT-qPCR assay in order to discard any variation in PbTUBE3 gene expression, confirming in this way that this gene can be used as normalizer under the experimental conditions carried out in this work