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Since the low-molecular-weight antioxidants glutathione (GSH) and thioredoxin (Trx-Red or Trx-SH) are cofactors of various enzymes, we may include them in the first line of antioxidant defense (Ighodaro and Akinloye 2018)

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The following day adipocytes were incubated with 1:500 secondary antibody Goat anti-Mouse IgG Alexa 488 (Thermo Fisher Scientific) in 16% (v/v) goat serum with 0.3% (v/v) Triton X-100 for 1 hour at room temperature

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Cancer Immunol Res 7(10):16051618 Xia HL, Lv Y, Xu CW et al (2017) MiR-513c suppresses neuroblastoma cell migration, invasion, and proliferation through direct targeting glutaminase (GLS)