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Hence, we conducted a genome-scale screening in hepatocytes using a library targeting over 18,000 protein-coding genes to identify genes whose deficiency culminated in the LDLR high phenotype

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cDNAs were diluted 10 times in water, and 2 L were used for qPCR, which was performed in MicroAmp Endura Plate Optical 96 well fast clear (Applied Biosystems, # 4483485), using the Power SYBR Green PCR Master Mix (Applied Biosystems, # 4367659) in a final volume of 10 L

[162] The Santos family, along with the San Diego district attorney, sued to stop the commutation, claiming that it violated Marsy's Law

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Methods: In vitro resistance selection was conducted in the presence of G418 and gradually increasing concentrations of AT-511 (free base of BEM)