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Investigations are guided by clinical suspicion rather than routine panels: for example, thyroid function tests and ferritin may be checked if symptoms suggest deficiency or thyroid disease, while an androgen profile may be appropriate in women presenting with menstrual irregularity, hirsutism, or acne

Studies in animals show that systemic administration produces healing effects at distant sites

Prevent foam by avoiding any shaking motion and injecting water slowly down the vial wall