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Cell Culture The human colon cancer cell lines HCT116 and SW620, purchased from American Type Culture Collection (Manassas, VA, USA), were cultured in RPMI medium and DMEM/F12 (HyClone, Logan, UT, USA) respectively, supplemented with 10% foetal bovine serum (HyClone) and 1% penicillin and streptomycin

Through modulation of upstream factors like AMPK, ammonia inhibits mTOR activity, thereby reducing protein synthesis (106)

Classical homocystinuria, an autosomal-recessive disease, is caused by CBS deficiency

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They found low magnesium levels in 95% of the children. (p