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Substrate (e.g., glucose) consumption and oxygen consumption continue, and will actually increase, because the protons are not being pushed out against a gradient and just flow right back in without doing any work to make ATP

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J Neurochem 97:1634-1658 Ballatori N, Krance SM, Notenboom S, Shi S, Tieu K, Hammond CL (2009) Glutathione dysregulation and the etiology and progression of human diseases

It is naturally produced by the liver and comprises the amino acids cysteine, glycine, and glutamine

The total cell lysate was cleared by centrifugation and supplemented with 25 L per liter culture OD 600 of BioLock avidin (IBA Lifesciences, Gttingen, Germany) to mask D-biotin from the culture medium and/or endogenous biotinylated proteins from the host cell, followed by sterile-filtration