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10.1523/JNEUROSCI.0616-08.2008 J

Pellets were thawed on ice and lysed in 2X lysis buffer (100 mM TrisHCl pH 7.5, 300 mM NaCl, 2 mM EDTA, 2% (v/v) IGEPAL CA-630, 1% sodium deoxycholate, 0.5 mM TCEP (Tris(2-carboxyethyl)phosphine hydrochloride) with EDTA-free Protease Inhibitor Cocktail (Sigma-Aldrich) for 20 min on ice

Mahjoum, E

doi: 10.1146/annurev-pharmtox-010818-021524 79

Notwithstanding mostly SCFAs uptake, signaling and functions occurring in the gut, associative studies show that SCFAs have an important role in human metabolism, the immune system and the entire organism, being able to cross the BBB [192, 193]