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smegmatis infected THP-1 cells with PAF C-16 at concentrations higher or lower than 1 g/ml [0.001 g (1.90 nM), 0.01 g (19.09 nM), 0.1 g (190.9 nM), 5 g (9.5 M), and 10 g/ml (19.09 M)] for 24 h showed no significant inhibitory effect on the growth of intracellular M

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After 4 h of infection, the cells were washed thrice with 1X PBS to remove the extracellular bacteria and replenished with fresh media

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