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Flow cytometry Cells, at a density of 5 10 5 cells per well, were plated into a 6-well plate and incubated in a cell incubator (37 C, 5% CO 2 ) until reaching 8090% confluence, and the concentration of LAAM GSH-CQDs was 10 g mL 1

Proper Rest: Allow your horse adequate rest and controlled exercise during recovery

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10.1016/j.molmed.2010.09.002 19 DattaJ.MajumderS.KutayH.MotiwalaT.FrankelW.CostaR.et al (2007)

Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) Total RNAs were isolated from ovarian tissues or GCs using Trizol regent (ThermoFisher, USA) and reverse transcribed into cDNA using M-MLV reverse transcriptase (Promega, Shanghai, China)

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