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Research indicates that MLT may inhibit ferroptosis through the Sirt6/Nrf2/HO-1 pathway, thus exerting antidepressant effects

(A) Intracellular IL-1b staining, (B) Intracellular IL-10 staining, (C) Intracelllular IL-1b and (D) IL-10 staining in macrophages from LPS pre-treated animals, (E) Thioltracker thiol-dependent fluorescence, (F) mitochondrial membrane potential, (G) mitochondrial superoxide production, (H) ATP and (I) GSH levels in peritoneal lavage, measured immediately after isolation

Morona, R., Purins, L., Tocilj, A., Matte, A

Excess tissues between hair follicles were removed by digestion with collagenase II (Gibco, Grand Island, NY, USA) at room temperature for about 30 min