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Although zebularine and 5AC are equally potent as small oligodeoxynucleotides, the potency of zebularine is approximately one-tenth of that of 5AC in inducing demethylation when used as a single agent ( 4.1.3 Guadecitabine Guadecitabine (SGI-110), also a second-generation DNA methyltransferase inhibitor, is a dinucleotide molecule linked by a phosphodiester bond between DAC and deoxyguanosine ( Loss of DNMT1 expression results in prolonged activation and promoter DNA demethylation of tumor-testis antigens, including the melanoma-associated antigen (MAGEA)-1 and MAGEA3 genes in human fibroblasts