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We utilized Superscript III reverse transcriptase enzyme (Invitrogen) with random hexamer primers and 3 g of RNA from each sample to produce cDNA according to the manufacturers protocol

| 05.15.26 | 12 min read

The mechanism underlying the barrier-protective effect of Lactobacillus in IBD The effect of Lactobacillus on the immunological barrier The effect of Lactobacillus on immune cells As the first line of defense against microbiota, intestinal mucosa harbors numerous immune cells consisting of lymphocytes, dendritic cells, granulocytes, etc

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