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In comparison, obvious fluorescence signal was observed in the cytoplasm at pH 6.5, suggesting that the self-assembled Gi-F-CAA possessed a higher internalization capacity by cellular endocytosis

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NAPQI-GST Formation by CYP3A4 CYP3A4 Supersomes (1 mg/mL protein, 125 pmol/mL CYP3A4 final concentration) and APAP (100 M) were incubated (37C and 500 rpm) in the presence of NADPH (1 mM in phosphate buffer)

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Compared to DBA baseline measures of these transcripts, ALA treatment enhanced Ho-1 expression, lowered Nos-2 and Gfap expression and led to slightly lower Cp mRNA levels (2-fold increase versus 4-fold increase in DBA) (Figures 2 and 7A)

Indeed, iGPX4 may be a promising target for NAFLD therapy as activated iGPX4 interacts with the canonical cGPX4 by transforming cGPX4 from enzymatically active monomers to enzymatically inactive oligomers in response to lipid stress, which exacerbates ferroptosis (Tong et al., 2022)