glutathione sepharose column sigma Sepharose™ 4B Cytiva 17-0756-04, pack of 300 mL molecular pull glutathione sepharose beads sigma How

Proximity ligation assay Cells were grown on glass cover slips for 36 h, fixed with 3.7% paraformaldehyde for 10 min, and permeabilized with 0.1% Triton X-100 in PBS for 5 min

siRNA knockdowns Cells were transfected with 20 nM of the indicated siRNAs using Opti-MEM (Thermo, 31985062) and RNAimax (Thermo, 13778150) according to the manufacturers protocol

In 2010, they synthesized a diselenide-linked polyurethane block copolymer, capped with polyethylene glycol (PEG), which served as a nanocarrier [60]

Vojnov et al., 2010

This conversion rate can be used for estimation of NADPH and NADP + concentrations in fresh blood based on levels measured in frozen sample

Total GSH is measured at an absorbance of 412 nm as the oxidized GSSG is reduced to two GSHs by NADPH, Glutathione reductase, and the reduced GSH and GSH present in the existing sample react with DTNB to form yellow TNB