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Both screening approaches led to the identification of hit compounds that were able to reduce NS3-NS5 binding in vitro in a dose-dependent manner, as measured by an ELISA-based assay

Galon J, Mlecnik B, Bindea G, Angell HK, Berger A, Lagorce C, Lugli A, Zlobec I, Hartmann A, Bifulco C, Nagtegaal ID, Palmqvist R, Masucci GV, Botti G, Tatangelo F, Delrio P, Maio M, Laghi L, Grizzi F, Asslaber M, D'Arrigo C, Vidal-Vanaclocha F, Zavadova E, Chouchane L, Ohashi PS, Hafezi-Bakhtiari S, Wouters BG, Roehrl M, Nguyen L, Kawakami Y, Hazama S, Okuno K, Ogino S, Gibbs P, Waring P, Sato N, Torigoe T, Itoh K, Patel PS, Shukla SN, Wang Y, Kopetz S , Sinicrope FA, Scripcariu V, Ascierto PA, Marincola FM, Fox BA, Pags F

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For more on this topic, be sure to check out my recent podcast with methylation expert Dr

Protein kinase C (PKC)-mediated phosphorylation of heat shock protein family B (small) member 1 (HSPB1/HSP25/HSP27) limits the cytoskeleton-mediated iron uptake and therefore limits ferroptotic cancer cell death