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Visualizing the site and dynamics of IgG salvage by the MHC class I-related receptor, FcRn

Figure 8

Her team needed a reliable, reproducible method to measure ROS output while screening compounds to decrease ROS levels

Flow cytometry and sorting For surface staining cells were incubated at 48 C, in the dark with fluorochrome-conjugated CD19, CD23, CD21-CD35 or CD35, IgD, IgM, CD1d, CD24, CD93, CD95, and GL-7 diluted 1:200 (refer to Supplementary Data 4 for details)

[4] Glutathione Depletion and Supplementation in Medical Conditions Extensive research in various specialties has shown that many human diseases are associated with low glutathione levels

The small size of the polyhistidine tag renders it less immunogenic than other larger tags