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Tissue Engineering and Regenerative Medicine 19 (4), 823837

Sixty adult male albino rats were allocated into ten groups, comprising six rats each, for the study

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Membranes were incubated with primary antibodies against GST alpha, mu and pi (dilution 1:4000 for GST alpha, 1:2000 for GST pi and 1:1000 for GST mu) for 3 hours followed by treatment with secondary antibodies over night in +4C

Sections of 7 m were cut to perform H&E staining with Leica ST5010 Autostainer XL

Baseline data including liver Stiffness (LS), spleen stiffness (SS), liver biopsy findings as well as demographic data were included