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doi: 10.1016/j.molp.2019.04.002

Therefore, BME itself is not supposed to be a substrate for TurboID, although strong biotinylation was observed in living cells (Fig

The cells were pipetted into wells in a 96-well plate in triplicate, pelleted down by centrifuging for 3 min at 2700 x g , and resuspended using 5 M R110cl