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how they translate across routes is an open research question, not a solved one

Fluorescence microscopy For imaging, yeast strains tagged with fluorescent markers were cultured in nutrient-rich medium (YPD) and were treated with 300 M IAA for 8 h for conditional Vma2 degradation or shifted to nitrogen starvation (SD-N) medium for 2 h

If left untreated, it can lead to an increased risk of serious health problems, including permanent nerve damage

This allows researchers to isolate metabolic signaling effects without confounding endocrine responses, making AOD 9604 a useful model for studying localized metabolic regulation