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However, it is notoriously difficult to recapitulate these levels ex vivo, probably due to the prolonged differentiation process or absence of physiological stimuli

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Routine maintenance involves plating them at approximately 20000 cells/cm, replacing medium daily or every other day, and passaging twice weekly avoiding full confluency

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This will provide a more comprehensive understanding of how non-coding RNAs modulate fibrosis and AF, offering a novel approach to treating age-associated AF