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For systemic effects such as gut healing, systemic anti-inflammation, or neurological support, subcutaneous injection into the abdominal area is typically preferred

To detect MIM in the EV fractions from FBS, the PVDF membrane was incubated with anti-MIM antibody in Can Get Signal Solution 1 (TOYOBO, #NKB-201) overnight at 4 C and then with HRP-conjugated anti-mouse IgG antibody in Can Get Signal Solution 2 (TOYOBO, #NKB-301) overnight at 4 C

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Mol Nutr Food Res 65, e2100606.Google Scholar Wu, LY, Kan, CN, Cheah, IK et al