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2.4.8 DNA extraction from homogenized tissue Total DNA was extracted from intestinal mucosa and lymph nodes as previously described ( 2.4.9 qPCR for identification of MAP DNA from tissue and fecal samples To detect MAP genomes in DNA samples, a two-duplex real-time qPCR assay targeting IS900 and F57 was employed ( 2.5 Ex-vivo trial to evaluate the functionality of effector immune cells in killing wild-type MAP 2.5.1 Preparation of antigen presenting cells to prime effector immune cells The ex vivo trial was designed and conducted as previously described ( Figure 1D )