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DMSO is known to cause malformation in rodent embryos 17 , and we found that most of the zebrafish embryos treated with 5% (v/v) DMSO from 1.5 h post-fertilisation (hpf) died before 24 hpf, while 4 out of 27 embryos survived with severe malformation (Fig

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It is believed that the water molecules adjust to compensate for the loss of some hydrogen bonds and the formation of the weaker hexane-water attractions by forming new hydrogen bonds and acquiring a new arrangement

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