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The antioxidant mechanisms of edaravone are: enhancement of prostacyclin production, hydroxyl radical trapping and quenching of active oxygen (Sawada, 2017) (Table 1)

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Analysis of glutathione synthetase activity in cultured amniocytes or chorionic villi [30]

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After treatment, the cells were washed twice with HBSS and incubated in 2 mL of fresh culture medium without FBS