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Plates were preloaded with 10 l of laccase dilutions prepared in 20 mM citratephosphate buffer pH 7.0 as follows: 0.28 g ml 1 (LacAnc100) and 0.36 g ml 1 (LaccID) for ABTS, 1.4 g ml 1 (LacAnc100) and 1.8 g ml 1 (LaccID) for DMP, 3.4 g ml 1 (LacAnc100) and 4.0 g ml 1 (LaccID) for guaiacol, 1.7 g ml 1 (LacAnc100) and 8.1 g ml 1 (LaccID) for sinapic acid and 340 g ml 1 (LacAnc100) and 405 g ml 1 (LaccID) for p -coumaric acid

It can be primary or secondary to conditions like chronic lymphocytic leukemia

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