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coli BL21(DE3) cys cells cotransformed with plasmids pCGPx3M and pMazF (TaKaRa) were grown at 37C in 400 mL of MM medium (modified M9 minimal medium) supplemented with ampicillin (100 g/mL), kanamycin (25 g/mL), chloramphenicol (25 g/mL) and Cys (50 g/mL) on a shaker

Ascorbic Acid (Vitamin C): A powerful antioxidant that helps brighten skin, fade dark spots, and stimulate collagen production for a brighter, more youthful complexion

2019 May 14;39(5):BSR20182099

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To confirm this, we performed uniformly 13 C-labeled palmitic acid ([U- 13 C]PA) ( m + 16) flux analysis in glucose-deprived cells cultured in delipidated medium, in which PA ( m + 16)-derived acetyl-CoA ( m + 2) enters the TCA cycle and produces [ 13 C]citrate ( m + 2) and [ 13 C]malate ( m + 2) (Supplementary Fig