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Mutant KRAS increases the expression of glucose transporter 1 (GLUT1) and rate-limiting glycolytic enzymes, including hexokinases, phosphofructokinase 1 (PFK1), and lactate dehydrogenase A (LDHA), promoting glycolytic activity and increasing lactate production [17, 18]

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After separating the gel from the bottom of the wells, the diameter of the collagen gel was measured using analysis software (BZ-X800E Analyzer

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