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The V3+V4 region of the 16S rRNA gene was amplified by PCR using upstream and downstream primers 338F 5-ACTCCTACGGGAGGCAGCA-3 and 806R 5-GGACTACHVGGGTWTCTAAT-3

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Correlation analysis revealed that Staphylococcus was correlated with a wide range of genera in the gut, including SCFAs producers like Butyricicoccus, Monoglobus, Clostridia_UCG_014, Prevotellaceae_UCG_001 , Parabacteroides , etc