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Physiol Biochem Zool 89:462472 Burraco P, Valds AE, Johansson F, Gomez-Mestre I (2017) Physiological mechanisms of adaptive developmental plasticity in Rana temporaria Island populations

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DMEM-F12) and phosphate-buffered saline (PBS) buffer, were purchased from ZETA life (USA), as was the cell counting kit-8 (CCK-8)

Trigonelline is an NAD+ precursor that improves muscle function during ageing and is reduced in human sarcopenia