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The Nrf2 pathway has been referred to as the master regulator of antioxidant, detoxification and cell defense gene expression

This event is associated with augmented lipid synthesis, thus, preserving mature oligodendrocytes viability, and protecting myelin through the modulation of cellular lipid metabolism

Materials and methods Cell culture Cholangiocarcinoma cell lines KKU-M213C and KKU-M213 were maintained in Dulbeccos modified Eagle medium (DMEM) (Gibco, Waltham, MA, USA), and cholangiocarcinoma cell lines SNU308, SNU478, SNU1196, and HuccT1 were maintained in RPMI1640 medium (Gibco, Waltham, MA, USA)

FIGURE 1 Material and methods Chemicals All standard chemicals used were purchased from verified sources

The transcript data used in this study are available in the MANE database (v1.3) 55