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On the basis of the evidence presented above, the major objectives of the present study were (1) to determine the effects of GSHee on ventilatory performance in freely-moving adult rats, and (2) to determine whether the prior administration of GSHee is able to ameliorate the negative effects of fentanyl on breathing and gas-exchange in freely-moving adult rats

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Alternatively, cell viability was determined by Cell Titer-Glo (CTG) assay (Promega) plating 5000 cells/96-well in 100 l media, treatment of cells the next for 48 h followed by Cell Titer-Glo (CTG) assay (Promega) according to the manufacturers instructions

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